Applicant:
Prof.Dr. Georg Auburger
Sektion Molekulare Neurogenetik
Klinik für Neurologie, Haus 26
Theodor Stern Kai 7, J.W. Goethe Universität,

60590 Frankfurt am Main
Tel: 069-6301-7428 FAX: 069-6301-7142
E-mail: auburger@em.uni-frankfurt.de

To elucidate the pathogenesis of familial spastic paraplegia type SPG3A it is important to understand the mutant disease protein atlastin-1 with regard to subcellular localisation at cell membranes, to its network of protein interactors, and its function as a GTPase. Using the yeast-2-hybrid-technique, we have identified an interactor-candidate, which exhibits several similarities with the SPG disease protein spastin. We plan to study its tissue expression and subcellular localization, to generate cDNA-clones of full-length with tag-markers as well as antibodies, and use them to perform colocalization and coimmunoprecipitation analyses with respect to atlastin-1 and this interactor-canidate. Hopefully, the results will shed light on the biological pathways between different disease proteins of spastic paraplegia.

For the results look to the following publications:

<link typo3 fileadmin projekte eva_luther_final_presentation_07.ppt download file>Powerpoint-Datei EvaLutherFinalPresentation07.ppt)

<link typo3 fileadmin projekte diplomarbeit_miguel_barrera.pdf download file>Diplomarbeit Miguel Barrera.pdf).

<link typo3 fileadmin projekte davidnonis_atlastin_co-ip_and_antibody.ppt download file>Datei David Nonis Atlastin Co-IP and Antibody.ppt).