How to charcterize spezific interaktion partner of Atlastin1


Prof.Dr. Georg Auburger
Sektion Molekulare Neurogenetik
Klinik für Neurologie, Haus 26
Theodor Stern Kai 7, J.W. Goethe Universität,

60590 Frankfurt am Main
Tel: 069-6301-7428 FAX: 069-6301-7142

To elucidate the pathogenesis of familial spastic paraplegia type SPG3A it is important to understand the mutant disease protein atlastin-1 with regard to subcellular localisation at cell membranes, to its network of protein interactors, and its function as a GTPase. Using the yeast-2-hybrid-technique, we have identified an interactor-candidate, which exhibits several similarities with the SPG disease protein spastin. We plan to study its tissue expression and subcellular localization, to generate cDNA-clones of full-length with tag-markers as well as antibodies, and use them to perform colocalization and coimmunoprecipitation analyses with respect to atlastin-1 and this interactor-canidate. Hopefully, the results will shed light on the biological pathways between different disease proteins of spastic paraplegia.

For the results look to the following publications:

<link typo3 fileadmin projekte eva_luther_final_presentation_07.ppt download file>Powerpoint-Datei EvaLutherFinalPresentation07.ppt)

<link typo3 fileadmin projekte diplomarbeit_miguel_barrera.pdf download file>Diplomarbeit Miguel Barrera.pdf).

<link typo3 fileadmin projekte davidnonis_atlastin_co-ip_and_antibody.ppt download file>Datei David Nonis Atlastin Co-IP and Antibody.ppt).